1BRS_A_D

• Mutation(s) in PDB: RA87A
• Mutation(s) cleaned: RA85A
• Location: SUP
• Hold out type:
• Hold out protein(s): 1BRS_A_D,1B2U_A_D,1B2S_A_D,1B3S_A_D,1X1W_A_D,1X1X_A_D
• Affinity mutant: 1.2E-10
• Affinity wild-typen: 1E-14
• Reference: 7739054
• Protein 1: Barnase
• Protein 2: Barstar
• Method: ITC
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): 1.4E+08
• Kon wild-type (M^-1s^-1): 3.7E+08
• Koff mutatant (M^-1s^-1): 1.7E-02
• Koff wild-type (M^-1s^-1): 3.73E-06
• ΔH mutant (kcal mol^-1)): -17.3
• ΔH wild-type (kcal mol^-1)): -19.3
• ΔS mutant (cal mol^-1 K^-1): -12.7
• ΔS wild-type (cal mol^-1 K^-1): -1.01