SKEMPI v2.0
Structural database of Kinetics and Energetics of Mutant Protein Interactions
1BRS_A_D
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- Mutation(s) in PDB:
RA87A
- Mutation(s) cleaned: RA85A
- Location: SUP
- Hold out type:
- Hold out protein(s): 1BRS_A_D,1B2U_A_D,1B2S_A_D,1B3S_A_D,1X1W_A_D,1X1X_A_D
- Affinity mutant: 1.2E-10
- Affinity wild-typen: 1E-14
- Reference: 7739054
- Protein 1: Barnase
- Protein 2: Barstar
- Method: ITC
- Temperaute: 298 K
- Kon mutatant (M-1s-1): 1.4E+08
- Kon wild-type (M-1s-1): 3.7E+08
- Koff mutatant (M-1s-1): 1.7E-02
- Koff wild-type (M-1s-1): 3.73E-06
- ΔH mutant (kcal mol-1)): -17.3
- ΔH wild-type (kcal mol-1)): -19.3
- ΔS mutant (cal mol-1 K-1): -12.7
- ΔS wild-type (cal mol-1 K-1): -1.01