SKEMPI v2.0
Structural database of Kinetics and Energetics of Mutant Protein Interactions
1BRS_A_D
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- Mutation(s) in PDB:
RA87A,
WD38F
- Mutation(s) cleaned: RA85A,WD38F
- Location: SUP,COR
- Hold out type:
- Hold out protein(s): 1BRS_A_D,1B2U_A_D,1B2S_A_D,1B3S_A_D,1X1W_A_D,1X1X_A_D
- Affinity mutant: 1.45E-09
- Affinity wild-typen: 1E-14
- Reference: 7739054
- Protein 1: Barnase
- Protein 2: Barstar
- Method: SFFL
- Temperaute: 298 K
- Kon mutatant (M-1s-1): 1.9E+08
- Kon wild-type (M-1s-1): 3.7E+08
- Koff mutatant (M-1s-1): 2.75E-01
- Koff wild-type (M-1s-1): 3.73E-06
- ΔH mutant (kcal mol-1)): None
- ΔH wild-type (kcal mol-1)): None
- ΔS mutant (cal mol-1 K-1): None
- ΔS wild-type (cal mol-1 K-1): None