1BRS_A_D

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  • Mutation(s) in PDB: RA87A, DD39A
  • Mutation(s) cleaned: RA85A,DD39A
  • Location: SUP,COR
  • Hold out type:
  • Hold out protein(s): 1BRS_A_D,1B2U_A_D,1B2S_A_D,1B3S_A_D,1X1W_A_D,1X1X_A_D
  • Affinity mutant: 1.85E-09
  • Affinity wild-typen: 1E-14
  • Reference: 7739054
  • Protein 1: Barnase
  • Protein 2: Barstar
  • Method: ITC
  • Temperaute: 298 K
  • Kon mutatant (M-1s-1): 1.6E+08
  • Kon wild-type (M-1s-1): 3.7E+08
  • Koff mutatant (M-1s-1): 3E-01
  • Koff wild-type (M-1s-1): 3.73E-06
  • ΔH mutant (kcal mol-1)): -13.4
  • ΔH wild-type (kcal mol-1)): -19.3
  • ΔS mutant (cal mol-1 K-1): -5.03
  • ΔS wild-type (cal mol-1 K-1): -1.01