1TM1_E_I

• Mutation(s) in PDB: RI65A
• Mutation(s) cleaned: RI46A
• Location: INT
• Hold out type: Pr/PI
• Hold out protein(s): Pr/PI
• Affinity mutant: 3.62E-10
• Affinity wild-typen: 2E-12
• Reference: 10065709
• Protein 1: Subtilisin BPN
• Protein 2: Chymotrypsin inhibitor 2
• Method: SFFL
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): 9.6E+05
• Kon wild-type (M^-1s^-1): 3.1E+06
• Koff mutatant (M^-1s^-1): 3.47E-04
• Koff wild-type (M^-1s^-1): 6.1E-06
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None