1TM1_E_I

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  • Mutation(s) in PDB: RI67A
  • Mutation(s) cleaned: RI48A
  • Location: SUP
  • Hold out type: Pr/PI
  • Hold out protein(s): Pr/PI
  • Affinity mutant: 2.78E-10
  • Affinity wild-typen: 2E-12
  • Reference: 10065709
  • Protein 1: Subtilisin BPN
  • Protein 2: Chymotrypsin inhibitor 2
  • Method: SFFL
  • Temperaute: 298 K
  • Kon mutatant (M-1s-1): 1.13E+06
  • Kon wild-type (M-1s-1): 3.1E+06
  • Koff mutatant (M-1s-1): 3.15E-04
  • Koff wild-type (M-1s-1): 6.1E-06
  • ΔH mutant (kcal mol-1)): None
  • ΔH wild-type (kcal mol-1)): None
  • ΔS mutant (cal mol-1 K-1): None
  • ΔS wild-type (cal mol-1 K-1): None