1B2S_A_D

• Mutation(s) in PDB: AA27K, AD43T
• Mutation(s) cleaned: AA27K,AD43T
• Location: SUP,RIM
• Hold out type:
• Hold out protein(s): 1BRS_A_D,1B2U_A_D,1B2S_A_D,1B3S_A_D,1X1W_A_D,1X1X_A_D
• Affinity mutant: 1E-14
• Affinity wild-typen: 1.57E-10
• Reference: 7739054
• Protein 1: Barnase
• Protein 2: Barstar
• Method: SFFL
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): 3.7E+08
• Kon wild-type (M^-1s^-1): 4.3E+07
• Koff mutatant (M^-1s^-1): 3.7E-06
• Koff wild-type (M^-1s^-1): 6.8E-03
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None