1BRS_A_D

• Mutation(s) in PDB: ED76A
• Mutation(s) cleaned: ED74A
• Location: RIM
• Hold out type:
• Hold out protein(s): 1BRS_A_D,1B2U_A_D,1B2S_A_D,1B3S_A_D,1X1W_A_D,1X1X_A_D
• Affinity mutant: 5.3E-10
• Affinity wild-typen: 1.32E-10
• Reference: Water-mediated interaction at a protein-protein interface, Chemical Physics, Volume 307, Issues 2-3, 2004
• Protein 1: Barnase
• Protein 2: Barstar
• Method: SPR
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): 8.97E+05
• Kon wild-type (M^-1s^-1): 8.74E+05
• Koff mutatant (M^-1s^-1): 4.75E-04
• Koff wild-type (M^-1s^-1): 1.15E-04
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None