SKEMPI v2.0
Structural database of Kinetics and Energetics of Mutant Protein Interactions
1TM1_E_I
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- Mutation(s) in PDB:
RI67C
- Mutation(s) cleaned: RI48C
- Location: SUP
- Hold out type: Pr/PI
- Hold out protein(s): Pr/PI
- Affinity mutant: 4.71E-10
- Affinity wild-typen: 2E-12
- Reference: 10065709
- Protein 1: Subtilisin BPN
- Protein 2: Chymotrypsin inhibitor 2
- Method: SFFL
- Temperaute: 298 K
- Kon mutatant (M-1s-1): 6.4E+05
- Kon wild-type (M-1s-1): 3.1E+06
- Koff mutatant (M-1s-1): 3.03E-04
- Koff wild-type (M-1s-1): 6.1E-06
- ΔH mutant (kcal mol-1)): None
- ΔH wild-type (kcal mol-1)): None
- ΔS mutant (cal mol-1 K-1): None
- ΔS wild-type (cal mol-1 K-1): None