1BRS_A_D
- Mutation(s) in PDB: ED80A
- Mutation(s) cleaned: ED78A
- Location: SUR
- Hold out type:
- Hold out protein(s): 1BRS_A_D,1B2U_A_D,1B2S_A_D,1B3S_A_D,1X1W_A_D,1X1X_A_D
- Affinity mutant: 2.64E-10
- Affinity wild-typen: 1.32E-10
- Reference: Water-mediated interaction at a protein-protein interface, Chemical Physics, Volume 307, Issues 2-3, 2004
- Protein 1: Barnase
- Protein 2: Barstar
- Method: SPR
- Temperaute: 298 K
- Kon mutatant (M-1s-1): 8.62E+05
- Kon wild-type (M-1s-1): 8.74E+05
- Koff mutatant (M-1s-1): 2.28E-04
- Koff wild-type (M-1s-1): 1.15E-04
- ΔH mutant (kcal mol-1)): None
- ΔH wild-type (kcal mol-1)): None
- ΔS mutant (cal mol-1 K-1): None
- ΔS wild-type (cal mol-1 K-1): None