1BRS_A_D

• Mutation(s) in PDB: KA27A
• Mutation(s) cleaned: KA25A
• Location: COR
• Hold out type:
• Hold out protein(s): 1BRS_A_D,1B2U_A_D,1B2S_A_D,1B3S_A_D,1X1W_A_D,1X1X_A_D
• Affinity mutant: 1.2E-10
• Affinity wild-typen: 1.3E-14
• Reference: 8494892
• Protein 1: Barnase
• Protein 2: Barstar
• Method: SFFL
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): 5.5E+07
• Kon wild-type (M^-1s^-1): 6E+08
• Koff mutatant (M^-1s^-1): 6.6E-03
• Koff wild-type (M^-1s^-1): 8E-06
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None