1TM1_E_I

• Mutation(s) in PDB: TI58D, EI60A
• Mutation(s) cleaned: TI39D,EI41A
• Location: COR,COR
• Hold out type: Pr/PI
• Hold out protein(s): Pr/PI
• Affinity mutant: 2.5E-12
• Affinity wild-typen: 7E-13
• Reference: 7947796
• Protein 1: Subtilisin BPN
• Protein 2: Chymotrypsin inhibitor 2
• Method: IASP
• Temperaute: 298(assumed) K
• Kon mutatant (M^-1s^-1): 4.3E+06
• Kon wild-type (M^-1s^-1): 5.5E+06
• Koff mutatant (M^-1s^-1): 1.07E-05
• Koff wild-type (M^-1s^-1): 3.9E-06
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None