1R0R_E_I

• Mutation(s) in PDB: LI18M, SI51N
• Mutation(s) cleaned: LI13M,SI46N
• Location: COR,SUR
• Hold out type: Pr/PI
• Hold out protein(s): Pr/PI
• Affinity mutant: 1.27E-11
• Affinity wild-typen: 2.94E-11
• Reference: Stephen Ming-teh Lu, PhD Thesis, Purdue University, 2000
• Protein 1: Subtilisin Carlsberg
• Protein 2: Turkey ovomucoid third domain
• Method: FL
• Temperaute: 294 K
• Kon mutatant (M^-1s^-1): -
• Kon wild-type (M^-1s^-1): -
• Koff mutatant (M^-1s^-1): -
• Koff wild-type (M^-1s^-1): -
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None