SKEMPI v2.0
Structural database of Kinetics and Energetics of Mutant Protein Interactions
1R0R_E_I
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- Mutation(s) in PDB:
EI10D,
AI15V,
YI20D,
RI21M,
KI29I,
GI32H,
NI36Y,
VI42A,
EI43H,
LI50F
- Mutation(s) cleaned: EI5D,AI10V,YI15D,RI16M,KI24I,GI27H,NI31Y,VI37A,EI38H,LI45F
- Location: RIM,COR,COR,RIM,SUR,SUP,RIM,SUR,SUR,SUR
- Hold out type: Pr/PI
- Hold out protein(s): Pr/PI
- Affinity mutant: 4.35E-07
- Affinity wild-typen: 2.94E-11
- Reference: Stephen Ming-teh Lu, PhD Thesis, Purdue University, 2000
- Protein 1: Subtilisin Carlsberg
- Protein 2: Turkey ovomucoid third domain
- Method: FL
- Temperaute: 294 K
- Kon mutatant (M-1s-1): -
- Kon wild-type (M-1s-1): -
- Koff mutatant (M-1s-1): -
- Koff wild-type (M-1s-1): -
- ΔH mutant (kcal mol-1)): None
- ΔH wild-type (kcal mol-1)): None
- ΔS mutant (cal mol-1 K-1): None
- ΔS wild-type (cal mol-1 K-1): None