2WPT_A_B

• Mutation(s) in PDB: DA33L
• Mutation(s) cleaned: DA30L
• Location: SUP
• Hold out type:
• Hold out protein(s): 2WPT_A_B,2VLP_A_B,2VLN_A_B,2VLQ_A_B,2VLO_A_B,1EMV_A_B,1FR2_A_B,2GYK_A_B
• Affinity mutant: 4.8E-11
• Affinity wild-typen: 1.5E-08
• Reference: 9718299
• Protein 1: Colicin E2 immunity protein
• Protein 2: Colicin E9 DNase
• Method: SFFL
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): 7.9E+07
• Kon wild-type (M^-1s^-1): 5E+07
• Koff mutatant (M^-1s^-1): 3.8E-03
• Koff wild-type (M^-1s^-1): 7.3E-01
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None