2WPT_A_B

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  • Mutation(s) in PDB: NA34V, RA38T, EA39H, RA42E
  • Mutation(s) cleaned: NA31V,RA35T,EA36H,RA39E
  • Location: COR,RIM,INT,SUR
  • Hold out type:
  • Hold out protein(s): 2WPT_A_B,2VLP_A_B,2VLN_A_B,2VLQ_A_B,2VLO_A_B,1EMV_A_B,1FR2_A_B,2GYK_A_B
  • Affinity mutant: 9.5E-11
  • Affinity wild-typen: 1.5E-08
  • Reference: 9718299
  • Protein 1: Colicin E2 immunity protein
  • Protein 2: Colicin E9 DNase
  • Method: SFFL
  • Temperaute: 298 K
  • Kon mutatant (M-1s-1): 1.45E+08
  • Kon wild-type (M-1s-1): 5E+07
  • Koff mutatant (M-1s-1): 1.3E-02
  • Koff wild-type (M-1s-1): 7.3E-01
  • ΔH mutant (kcal mol-1)): None
  • ΔH wild-type (kcal mol-1)): None
  • ΔS mutant (cal mol-1 K-1): None
  • ΔS wild-type (cal mol-1 K-1): None