3M63_A_B

• Mutation(s) in PDB: EA27A
• Mutation(s) cleaned: EA33A
• Location: SUR
• Hold out type:
• Hold out protein(s): 3M63_A_B,3M62_A_B,1S1Q_A_B,1XD3_A_B,2OOB_A_B
• Affinity mutant: 5.21E-07
• Affinity wild-typen: 1.75E-07
• Reference: 20427284
• Protein 1: Ubiquitin conjugation factor E4
• Protein 2: DSK2
• Method: ITC
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): -
• Kon wild-type (M^-1s^-1): -
• Koff mutatant (M^-1s^-1): -
• Koff wild-type (M^-1s^-1): -
• ΔH mutant (kcal mol^-1)): -7.3
• ΔH wild-type (kcal mol^-1)): -10.1
• ΔS mutant (cal mol^-1 K^-1): 4.36
• ΔS wild-type (cal mol^-1 K^-1): -2.68