3M63_A_B

• Mutation(s) in PDB: GB10F, QB11K, SB67Q, HB69V, VB71M
• Mutation(s) cleaned: GB8F,QB9K,SB65Q,HB67V,VB69M
• Location: RIM,SUR,RIM,COR,COR
• Hold out type:
• Hold out protein(s): 3M63_A_B,3M62_A_B,1S1Q_A_B,1XD3_A_B,2OOB_A_B
• Affinity mutant: 2.4E-07
• Affinity wild-typen: 1.75E-07
• Reference: 20427284
• Protein 1: Ubiquitin conjugation factor E4
• Protein 2: DSK2
• Method: ITC
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): -
• Kon wild-type (M^-1s^-1): -
• Koff mutatant (M^-1s^-1): -
• Koff wild-type (M^-1s^-1): -
• ΔH mutant (kcal mol^-1)): -2.5
• ΔH wild-type (kcal mol^-1)): -10.1
• ΔS mutant (cal mol^-1 K^-1): 21.8
• ΔS wild-type (cal mol^-1 K^-1): -2.68