2WPT_A_B

• Mutation(s) in PDB: KA20T, KA21T, RA24N, EA26D, GA27T, AA28S, TA29S, DA32E, DA33L, NA34V, RA38T, EA39H, RA42E, LA43M
• Mutation(s) cleaned: KA17T,KA18T,RA21N,EA23D,GA24T,AA25S,TA26S,DA29E,DA30L,NA31V,RA35T,EA36H,RA39E,LA40M
• Location: INT,SUR,RIM,RIM,SUP,RIM,RIM,SUR,SUP,COR,RIM,INT,SUR,INT
• Hold out type:
• Hold out protein(s): 2WPT_A_B,2VLP_A_B,2VLN_A_B,2VLQ_A_B,2VLO_A_B,1EMV_A_B,1FR2_A_B,2GYK_A_B
• Affinity mutant: 1.28E-14
• Affinity wild-typen: 1.47E-08
• Reference: 9268373
• Protein 1: Colicin E2 immunity protein
• Protein 2: Colicin E9 DNase
• Method: SFFL
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): -
• Kon wild-type (M^-1s^-1): -
• Koff mutatant (M^-1s^-1): -
• Koff wild-type (M^-1s^-1): -
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None