SKEMPI v2.0
Structural database of Kinetics and Energetics of Mutant Protein Interactions
1EMV_A_B
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- Mutation(s) in PDB:
DA51A
- Mutation(s) cleaned: DA49A
- Location: COR
- Hold out type:
- Hold out protein(s): 2VLP_A_B,2VLN_A_B,2VLQ_A_B,2VLO_A_B,1EMV_A_B,1FR2_A_B,2GYK_A_B,2WPT_A_B
- Affinity mutant: 3.53E-10
- Affinity wild-typen: 1.62E-14
- Reference: 9425068
- Protein 1: Colicin E9 immunity protein
- Protein 2: Colicin E9 DNase
- Method: SFFL
- Temperaute: 298 K
- Kon mutatant (M-1s-1): 1.73E+07
- Kon wild-type (M-1s-1): 1.13E+08
- Koff mutatant (M-1s-1): 6.11E-03
- Koff wild-type (M-1s-1): 1.83E-06
- ΔH mutant (kcal mol-1)): None
- ΔH wild-type (kcal mol-1)): None
- ΔS mutant (cal mol-1 K-1): None
- ΔS wild-type (cal mol-1 K-1): None