1FR2_A_B

• Mutation(s) in PDB: AA41E
• Mutation(s) cleaned: AA39E
• Location: COR
• Hold out type:
• Hold out protein(s): 2VLP_A_B,2VLN_A_B,2VLQ_A_B,2VLO_A_B,1EMV_A_B,1FR2_A_B,2GYK_A_B,2WPT_A_B
• Affinity mutant: 1.62E-14
• Affinity wild-typen: 5.46E-13
• Reference: 9425068
• Protein 1: Colicin E9 immunity protein
• Protein 2: Colicin E9 DNase
• Method: SFFL
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): 1.13E+08
• Kon wild-type (M^-1s^-1): 2.6E+07
• Koff mutatant (M^-1s^-1): 1.83E-06
• Koff wild-type (M^-1s^-1): 1.42E-05
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None