2SIC_E_I

• Mutation(s) in PDB: MI73E
• Mutation(s) cleaned: MI67E
• Location: COR
• Hold out type: Pr/PI
• Hold out protein(s): Pr/PI
• Affinity mutant: 6.9E-11
• Affinity wild-typen: 1.8E-11
• Reference: 8276767
• Protein 1: Subtilisin BPN
• Protein 2: Streptomyces subtilisin inhibitor
• Method: IASP
• Temperaute: 298 K
• Kon mutatant (M^-1s^-1): 1.9E+06
• Kon wild-type (M^-1s^-1): 6.5E+06
• Koff mutatant (M^-1s^-1): 1.1E-04
• Koff wild-type (M^-1s^-1): 9E-05
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None