4G0N_A_B

• Mutation(s) in PDB: RB59A, DA33A
• Mutation(s) cleaned: RB6A,DA33A
• Location: RIM,RIM
• Hold out type:
• Hold out protein(s): 4G0N_A_B,3KUD_A_B,1LFD_A_B,1GUA_A_B,1C1Y_A_B,1K8R_A_B,1HE8_A_B,1E96_A_B
• Affinity mutant: 5.61E-06
• Affinity wild-typen: 8.02E-08
• Reference: 15236966
• Protein 1: H-Ras1
• Protein 2: Raf-RBD
• Method: ITC
• Temperaute: 308 K
• Kon mutatant (M^-1s^-1): -
• Kon wild-type (M^-1s^-1): -
• Koff mutatant (M^-1s^-1): -
• Koff wild-type (M^-1s^-1): -
• ΔH mutant (kcal mol^-1)): -6.0
• ΔH wild-type (kcal mol^-1)): -8.6
• ΔS mutant (cal mol^-1 K^-1): 4.5454545455
• ΔS wild-type (cal mol^-1 K^-1): 4.55