4G0N_A_B

• Mutation(s) in PDB: TA35A
• Mutation(s) cleaned: TA35A
• Location: INT
• Hold out type:
• Hold out protein(s): 4G0N_A_B,3KUD_A_B,1LFD_A_B,1GUA_A_B,1C1Y_A_B,1K8R_A_B,1HE8_A_B,1E96_A_B
• Affinity mutant: 7.2E-06
• Affinity wild-typen: 5E-08
• Reference: 15236966
• Protein 1: H-Ras1
• Protein 2: Raf-RBD
• Method: SFFL
• Temperaute: 273 K
• Kon mutatant (M^-1s^-1): 7E+05
• Kon wild-type (M^-1s^-1): 3.55E+07
• Koff mutatant (M^-1s^-1): 5.1E+00
• Koff wild-type (M^-1s^-1): 1.7E+00
• ΔH mutant (kcal mol^-1)): None
• ΔH wild-type (kcal mol^-1)): None
• ΔS mutant (cal mol^-1 K^-1): None
• ΔS wild-type (cal mol^-1 K^-1): None