SKEMPI v2.0
Structural database of Kinetics and Energetics of Mutant Protein Interactions
1LFD_A_B
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- Mutation(s) in PDB:
KA52A
- Mutation(s) cleaned: KA39A
- Location: COR
- Hold out type:
- Hold out protein(s): 1LFD_A_B,4G0N_A_B,3KUD_A_B,1K8R_A_B,1HE8_A_B,1GUA_A_B,1C1Y_A_B
- Affinity mutant: 1.42E-05
- Affinity wild-typen: 1.94E-06
- Reference: 15197281
- Protein 1: RalGDS-RBD
- Protein 2: H-Ras1
- Method: SFFL
- Temperaute: 298 K
- Kon mutatant (M-1s-1): 2E+06
- Kon wild-type (M-1s-1): 7.7E+06
- Koff mutatant (M-1s-1): 2.83E+01
- Koff wild-type (M-1s-1): 1.49E+01
- ΔH mutant (kcal mol-1)): None
- ΔH wild-type (kcal mol-1)): None
- ΔS mutant (cal mol-1 K-1): None
- ΔS wild-type (cal mol-1 K-1): None